BestSends Guide

Email Marketing A/B Testing Guide

What to test, how long to run it, and how to tell a real winner from random noise on a small list.

Carlos Gil
Carlos Gil · Published · Updated · 9 min read
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The Power of Email A/B Testing

A/B testing is one of the most powerful tools in your email marketing arsenal, allowing you to make data-driven decisions rather than relying on gut instinct. When done correctly, A/B testing can lead to significant improvements in open rates, click-through rates, and conversions.

What is A/B Testing?

A/B testing (also called split testing) is a method of comparing two versions of an email to see which performs better. You send version A to one subset of subscribers and version B to another subset, then measure which variant drives more opens, clicks, or conversions.

What Elements to Test

Focus on testing one element at a time to get clear results. Here are the key elements that typically yield the most impactful insights:

Subject Lines

Test different lengths, tones, personalization, and question vs. statement formats.

Preview Text

Experiment with different preview text approaches that complement your subject line.

Send Time

Test different days of the week and times of day to find when your audience is most responsive.

CTA Buttons

Test button color, size, text, and placement to optimize click-through rates.

Email Layout

Compare single-column vs. multi-column layouts, or different content arrangements.

Content Length

Test short, concise emails against longer, more detailed messages.

Images

Compare different images, image sizes, or emails with images vs. text-only emails.

How to Run an Effective A/B Test

Follow these steps to ensure your tests deliver reliable results:

  1. 1. Set a Clear Objective

    Define what you're trying to improve (open rate, click rate, conversion rate).

  2. 2. Form a Hypothesis

    Create a hypothesis about what change might improve results and why.

  3. 3. Test One Variable at a Time

    Change only one element between versions to clearly identify what affects performance.

  4. 4. Use a Significant Sample Size

    Ensure each variant is sent to enough subscribers (typically at least 1,000 per variant).

  5. 5. Split Your Audience Randomly

    Ensure both groups have similar composition to avoid biased results.

  6. 6. Determine Statistical Significance

    Use statistical tools to confirm results are valid and not due to chance.

  7. 7. Document and Apply Learnings

    Record results and apply insights to future campaigns.

Common A/B Testing Mistakes to Avoid

  • Testing Too Many Variables

    When you change multiple elements at once, you can't determine which change impacted the results.

  • Using Too Small a Sample Size

    Small samples can lead to unreliable results that don't represent your broader audience.

  • Ending Tests Too Early

    Give your test enough time to gather sufficient data before drawing conclusions.

  • Not Accounting for External Factors

    Holidays, promotions, or news events can skew results if not considered.

  • Ignoring Statistical Significance

    A small difference in results might be due to chance rather than your tested variable.

Frequently asked questions

What should you A/B test in an affiliate email?

Test the subject line first, because opens cap everything downstream, then test where the recommendation sits in the body: early callout versus a link after the lesson. Do not test the disclosure away, and do not judge a subject line test on affiliate revenue, since the click is the metric the subject line actually controls.

Affiliate email marketing guide · Email marketing best practices

How many subscribers do you need for a valid email A/B test?

Roughly 1,000 recipients per variant. Below that, differences are usually noise. On a small affiliate list, test the offer and the audience segment instead of the wording, and let results accumulate over several sends rather than one campaign.

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Does A/B testing affect deliverability on affiliate campaigns?

The split itself does not, but link-heavy affiliate variants can. If one variant contains multiple redirect links and the other does not, treat inbox placement as part of the result and check spam placement, not just clicks.

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Carlos Gil

About the Author

Carlos Gil

Editor in Chief, BestSends

Marketing thought leader, LinkedIn Top Voice, and author of The End of Marketing (Wiley). Founder of The Best Network — including BestSends.com (email infrastructure), BestStacked.com (AI tools), and SenderlyAI.com.